Protocol for evaluating the in vitro effect of violet light-emitting diodes (LEDs) 410 nm ± 10 nm on yeast cultures

BACKGROUND: Candida spp and Malassezia cause superficial infections that may be resistant to conventional treatments. Violet light-emitting diodes (LEDs) therapy is a therapeutic alternative. PURPOSE: To describe the protocol for evaluating antifungal effect of violet LEDs 410 nm ± 10 on in vitro. PROTOCOL: are applied fungal suspension at fluences 61.13 J/cm2, 91.70 183.39 J/cm2. The isolates cultured 48 72 hours. Colony forming units (CFUs) quantified by visual counting percent culture plate occupancy digital analysis. Morphology assessed light microscopy Gram staining, yeast metabolism/function transmission electron microscopy, assessment reactive oxygen species, DNA fragmentation. DATA ANALYSIS: percentage inhibition calculated considering growth negative control condition yeasts dividing number pixels classified as colonies total plate. morphological functional aspects described intervention control. ANOVA test used compare mean percentages between three ESTIMATED RESULTS: We intend determine different spp. evaluation other species this should investigated.